直肠癌HER-2、COX-2和血清CEA表达及三者之间的相关性研究
潘贻飞;李权;卢山珊;万丽;项友群;金纯;郭贵龙;
摘要(Abstract)：
目的研究HER-2、COX-2和血清CEA在直肠癌中的表达及三者之间的相关性,并探讨其临床意义。方法采用免疫组化Envision二步法检测HER-2、COX-2蛋白在15例正常直肠癌旁组织、15例直肠腺瘤、50例直肠癌中的表达水平,采用化学发光免疫法测定术前50例直肠癌患者血清CEA值,分析HER-2、COX-2和血清CEA表达水平与临床病理因素的关系及三者之间的相关性。结果HER-2蛋白在正常直肠癌旁组织、直肠腺瘤及直肠癌中阳性表达率分别为0%、0%、6%,差异无显著性(P>0.05);COX-2蛋白在正常直肠癌旁组织、直肠腺瘤及直肠癌中阳性表达率分别为13.3%、40.0%、84.0%,差异显著(P<0.01)。50例直肠癌中血清CEA 26例高表达,24例低表达。直肠癌组织中HER-2蛋白表达与病理特点间无相关性;HER-2蛋白表达与COX-2蛋白表达、血清CEA之间均未见相关性(r=0.158,P>0.05;r=0.074,P>0.05);而COX-2蛋白阳性表达与淋巴结转移及Dukes分期相关(P<0.05);血清CEA表达与Dukes分期相关(P<0.05)。血清CEA与COX-2蛋白表达水平之间呈正相关(r=0.292,P<0.05)。结论HER-2蛋白在直肠癌中阳性表达率低,HER-2蛋白的表达在直肠癌中的作用可能很小,与血清CEA和COX-2无相关性,与直肠癌预后无关。COX-2和血清CEA呈相关性,均与直肠癌预后有关,两者在直肠癌发生、发展中可能存在某种联系。
关键词(KeyWords)： 直肠肿瘤;基因表达;受体,表皮生长因子;COX-2;癌胚抗原;免疫组织化学
基金项目(Foundation):
作者(Authors): 潘贻飞;李权;卢山珊;万丽;项友群;金纯;郭贵龙;
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