刘建波;李白羽;柯善保;李军涛

• 1:河南省人民医院肿瘤中心，河南 郑州 450000

摘要(Abstract)：

目的 探讨虎杖苷（polydatin，PD）对乳腺癌细胞放疗增敏的影响及可能作用机制。方法 MTT法检测不同浓度PD（5、10、20、40和80μmol/L）对人乳腺癌MCF-7细胞增殖的影响，克隆形成实验检测细胞放疗敏感性。将MCF-7细胞随机分为对照组（常规培养）、放疗组（2 Gy放射线）、LiCl组（2 Gy放射线+200μmol/L LiCl）、PD组（2 Gy放射线+20μmol/LPD）和PD+LiCl组（2 Gy放射线+20μmol/L PD+200μmol/L LiCl）。流式细胞术检测细胞凋亡率及细胞周期变化，Western blot法检测细胞中β-catenin、细胞周期素D1（cyclinD1）、B细胞淋巴瘤-2（B cell lymphoma-2，Bcl-2）和Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax）的表达水平。结果 不同浓度PD培养24、48和72 h对MCF-7细胞增殖均有抑制作用（均P<0.05）。20μmol/L的PD作用48 h可降低放疗（2、4、6和8 Gy）后细胞克隆形成率（plating efficiency，PE ；均P<0.05）。与放疗组比较，PD组细胞凋亡率、G₂/M期细胞比例和Bax蛋白表达均升高，G₀/G₁期细胞比例与β-catenin、cyclinD1和Bcl-2蛋白表达均降低（均P<0.05）；而PD+LiCl组可逆转PD改善的上述指标（均P<0.05）。结论 PD可将MCF-7细胞阻滞在G₂/M期，促进细胞凋亡，提高放疗敏感性，可能与抑制Wnt/β-catenin信号通路有关。

关键词(KeyWords)： 乳腺癌;虎杖苷;放疗增敏;增殖;凋亡

Abstract:

Keywords:

基金项目(Foundation): 河南省医学科技攻关计划项目(201702250)

作者(Authors): 刘建波;李白羽;柯善保;李军涛

DOI: 10.13267/j.cnki.syzlzz.2022.020

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